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BE 468

BE 468
Microbiological Engineering

Week # Topic Book Reading

1

Overview of MicrobiologyCell construction

DNA, RNA and protein synthesis

Ch. 2

Ch. 5

2

Bioenergetics

Major metabolic pathways

Strain development

Ch. 5

Ch. 5

Ch. 8

3

Enzyme kinetics

Competitive, noncompetitive, uncompetitive, and substrate inhibition

Immobilized Enzyme Systems

Design project proposal presentations

Ch. 3

4

Stirred Tank Reactors

Sterilization, medium and inoculum preparation

Batch fermentation and microbial growth

Fermentation Lab

Ch. 6

5

Continuous fermentation and kinetics

Optimization

Project Report 1 on microbial and medium characteristics

Ch. 6

6

Operating considerations

Two-vessel fermentors

First midterm exam

Ch. 9

7

Fed-Batch Systems

Recycled fermentation

Immobilized Cell Systems

Ch. 9

8

Aeration and oxygen transfer

Project Report 2 on bioreactor design


9

Spring Break

Ch. 10

10

Agitation

Scale-up strategies

Aeration Lab

Ch. 10

11

Product recovery and purification

Separation of insoluble products; Filtration, centrifugation, coagulation and flocculation

Project Report 3 on aeration and agitation

Ch. 11

12

Cell disruption; mechanical and non-mechanical methods

Ch. 11

13

Second midterm exam

Separation of soluble products; Liquid-liquid extraction, precipitation, adsorption, dialysis,

Ch.11

14

reverse osmosis, ultra filtration, cross flow ultra-filtration Chromatography, electrophoresis, electrodialysis

Project Report 4 on separation

Ch. 11

15

Finishing steps of purification; Crystallization, drying

Field Trip: Merck, Danville, PA

Ch. 11

16

Final Design project reports and presentations


17

Final Exam Week


Prerequisites: Phys 211 or 215; ABE 408 or Micro 201 and BMB 211

Class Time: MWF 11:15 AM – 12:05 PM in 106 Agricultural Engineering Building

Project Discussion or Lab Time: T 10:10 AM – 12:05 PM in 106 Agricultural Eng. Building

Required Text:

Michael L. Shuler and Fikret Kargi. 2002. Bioprocess Engineering : Basic Concepts. 2nd Edition. Prentice Hall, Englewood Cliffs, N.J.

Instructor:

Dr. Ali Demirci
231 Agricultural Eng. Bldg.
Phone: 814-863-1098
email: demirci@psu.edu

Grading:

Homework: 15%
Midterm Exams: 30%
Project reports/presentations: 40%
Final exam: 15%

Academic integrity, as defined by University Faculty Senate Policy 49-20, is the pursuit of scholarly activity free from fraud and deception and is an educational objective of this institution. Academic dishonesty includes, but is not limited to, cheating, plagiarizing, fabricating of information or citations, facilitating acts of academic dishonesty by others, having unauthorized possession of examinations, submitting work of another person or work previously used without informing the instructor, or tampering with the academic work of other students.