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BE 308

BE 308 - Engineering Elements of Biochemistry and Microbiology

Week # Topic Book Reading

1

Introduction

Organic chemistry applied to living system

  • Water
  • Carbohydrates

Ch. 1 & 2
Ch. 3

2
  • Lipids
  • Proteins
  • Enzyme

Lab #1: Analysis of sugars by HPLC

Ch. 3

3
  • Vitamins
  • Nucleic Acid

Lab #2: Coagulation of Milk Protein

Ch. 3

4

Basic Microbiology: An Engineer's Perspective

  • History of Microbiology
  • Microscopes

First Midterm (Sept. 26)

Ch. 1

Ch. 4

5
  • Prokaryotic Cells
  • Eukaryotic Cells
  • Viruses

Lab #3: Gram Staining of Microorganisms

Ch. 14
Ch. 9

6

Growth Kinetics and measurement of growth

  • Intrinsic and Extrinsic Factors

Lab #4: Preparation of Agar Plates and Dilution Tubes
Study Date

Ch. 6

7

Informatics and Genetic Information in Biological Systems

Ch. 7

  • Structures of DNA and RNA

Lab #5: Spread Plating

8

Synthesis of DNA

  • Transcription and Translation
  • Strain Development

Second Midterm

Lab #6: Enumeration of Colonies

Ch. 7

9

Energy Calculations in Microbial Bioenergetics

  • Energy and Carriers in Biological Systems
  • Energy Releasing Pathways: Glycolysis, Citric Acid Cycle
  • Oxidative Phosphorylation

Lab #7: Preparation of Fermentation Medium

Ch. 5

10

Industrial Microbiology

  • Raw materials
  • Fermentation Process
  • Bioreactors

Lab #8: Inoculation of Fermentation Medium

Ch. 30

11
  • Major Pathways
  • Organic Acids
  • Antibiotics

Lab #9: Analysis of Fermentation Broth by HPLC

Ch. 30

12
  • Enzymes, Amino Acids
  • Vitamins

Lab #10: Tour of Mushroom Facility

Ch. 30

13
  • Yeast as food
  • Mushrooms, etc.

Thanksgiving Holidays

 

14

Third Midterm

Environmental Microbiology

  • Sewage and Waste Water Treatment
  • Bioremediation
  • Biogas

Lab #11: BOD Analysis

 

Ch. 19

Ch. 28

15
  • Composting
  • Microbial leaching
  • Biofilm and biocorrosion

Lab #12: Waste Water Treatment Facility

Ch. 19

16

Final Exam Week

Instructor:

Dr. Ali Demirci
231 Agricultural Engineering Bldg.
Phone: 865-1098
E-mail: demirci@psu.edu

Grading:

First Midterm: 15%
Second Midterm: 15%
Third Midterm: 15%
Homework/Quizzes/Lab: 25%
Final Exam: 30%

Class Time: MWF 3:35 - 4:25 p.m. in 106 Agricultural Engineering Building

Lab Time: W 4:40 - 5:30 p.m. in 112 Agricultural Engineering Building

Required Text Book: Madigan, M.T. and Martinko, J.M. 2006. Brock Biology of Microorganisms. 11th Edition. Prince Hall. ISBN 0-13-144329-1

Academic integrity, as defined by University Faculty Senate Policy 49-20, is the pursuit of scholarly activity free from fraud and deception and is an educational objective of this institution. Academic dishonesty includes, but is not limited to, cheating, plagiarizing, fabricating of information or citations, facilitating acts of academic dishonesty by others, having unauthorized possession of examinations, submitting work of another person or work previously used without informing the instructor, or tampering with the academic work of other students.